What I was basically doing was cleaving a fluorescent DNA/RNA hybrid substrate with the enzyme RNase A, to find out what it's maximum fluorescence was at different concentrations. I can then compare my data to this standard curve to find out the concentration of product in all my runs. We'll see if it works. *crossfingers* I don't know why it wouldn't.
Below, are a few pictures of the lab and my labmates working hard. I wanted to document it, because I spend so little time in lab right now.
My labmates were working hard in lab today
My friend Tom was wrestling with a knotty problem
My friend inorganic/organic chemistry friend Tom in the lab on the 7th floor was wrestling with a knotty problem. I go visit him when I go to Emory. He's trying to separate an impurity from his compound of interest and he's tried just about everything, except an anion exchange column, which he is going to try next. Wish him luck!