Experiments This Week
Monday – no experiments
Tuesday – setup a simplified RNA FAM ligation reaction, along with positive control
Wednesday – poured and ran an 18% TBE-Urea gel of RNA FAM ligation reaction; Typhoon instrument had an error that prevented its imaging. Monitor screen would not appear. Emailed Joshua Lee about it.
Thursday – attended the SURE program poster session; Joshua Lee emailed to say the Typhoon was now working; setup of identical simplified FAM RNA ligation reaction
Friday – poured and ran an 18% TBE-Urea gel of RNA FAM ligation reaction; gel has similarities to 15% pre-cast gel run earlier – see page 95. Was concerned that since the FAM label was used by Raines and I was running the reaction under Shuman’s conditions (who used a radiolabel), the lack of salt (200 mM) in Shuman’s reactions may be causing an aberration with the FAM. Decided to run the same reaction under Raines and Shuman’s conditions to compare.
Purchases for the week = $13.66
My gels have been getting bubbles via detaching from the glass. This issue appears to have been solved by storing them more quickly after polymerization (~50 min).