Monday, October 27, 2014: Wrangling Nanopure and NSF
Wrangled all morning / early afternoon with fixing the Nanopure instrument. We think its filters went bad when construction workers were messing with the pipes and the water was brown for a day. Trying to order new filters, currently. I got transcripts for NSF sorted out. Finished updating my personal statement for the NSF. I cannot decide if it is better or not. Trying to update the proposal now. What a nightmare! I don't think I can make it sound perfect, no matter what I do.
Sunday, October 26, 2014: Relaxing continues
Read articles for class on Monday but not much else. I regret this. However, it was a nice, relaxing day.
Saturday, October 25, 2014: House hunting and relaxing
Played lots of Skyrim and went to look at houses with John. We are in the process of house hunting.
Thursday, October 23, 2014: Reading about ligases
Reading about ligases! I attempted to do an exhaustive search on ligases today from 9 am - 4 pm. So far, I've found one other ligase that may work in place of RtcB that has all the functions necessary in one enzyme. Grr. I'm going to try again tomorrow. I've still not answered the ribosome question.
Nanopure acting up. The resistivity on the Nanopure is dropping - our ultra-clean water system. There's no reason for this to happen. I'm mystified. Tried a lot of thoughts and called tech support. Going to try calibration if I can find the module.
Evening. Going to a Bible study this evening!
Nanopure acting up. The resistivity on the Nanopure is dropping - our ultra-clean water system. There's no reason for this to happen. I'm mystified. Tried a lot of thoughts and called tech support. Going to try calibration if I can find the module.
Evening. Going to a Bible study this evening!
Wednesday, October 22, 2014: Beginning Plans
Relief. I have been SOO happy that I passed the second year report on the 21st! For info about that, see this post here. Today, I began looking at the papers Dr. Weinert recommended for me - other ligases that I could use rather than RtcB. I found a problem with them. They leave a 2'-P on the RNA after ligation that has to be removed by another enzyme. Question: Can ribosomes read through this kind of transcript or must the P be removed?
Evening. I played a lot of Skyrim, wiping out a tower of bandits, one of witches and one of machine / automatons. I also went jogging.
Evening. I played a lot of Skyrim, wiping out a tower of bandits, one of witches and one of machine / automatons. I also went jogging.