Research Update: We Found One Source of the Problem!
I did activity assay on my protein this week and discovered that it's activity had decreased remarkably since last year, even under identical conditions. I talked with Dr. Weinert and discovered that even the most stable proteins only last 6 months at best. My protein is already a year old - even in -80C storage - it goes bad. So, I just have to isolate new!
The RNA I was using for the activity assay is also likely not in the best condition. It's phosphorylated on its 3' end and Dr. Weinert said that with their cyclic phosphates - which are different, but similar enough - they see degradation within a month. Mine are also a year old. So, I ordered new of those on Friday!
While listening to the speaker this Friday, experiments started coming together in my mind. I think I have a way to label my RNA substrate with a duel tag. With such a label, I will be able to confirm splice product without PCR, theoretically. It's *very* exciting!
But alas! Summer is ending and I have only two more weeks to get real things done in lab before focusing seriously on writing my second year report. And classes are about to start. I'm hoping I can get some key pieces of data that will help before then. Man, it'll be awesome when I'm done with everything! I look forward to diving back into research after the fall craziness ends.
What's going on in my corner of the science world? Want to know what the heck I do in lab or wonder what the life of a grad student is like? Need Orgo tutoring notes? Then this is the blog for you! Also, check out my FAQ about grad life and the Salaita lab. Look here for fun facts about lab members.
Rolling Statuses: Technical journal blog. Here you may discover what the daily life of a grad student looks like: day-to-day snippets of life, clutter, rolling statuses and unimportant fluff.
Progress Updates: Will include entries with more meaningful science.
Weekly lab report: My write-ups on what I did each week (I posted these publicly during my rotation but not as much now. That may change.)
Here is a link to collected writing, poster and presentation tips.
As of February 8, 2014 I have officially joined the Salaita lab!! Very exciting. Stay tuned for updates. "Micro Min" category equates to grad school journaling; most of these have moved to my status updates blog under Home tab. See "progress updates" on this blog for more important news.